Table 2. Illumina sequencing data and polymorphism statistics for five population samples of Lumnitzera racemosa.
QH | SY | KP | JH | KD | |
---|---|---|---|---|---|
Number of sequenced genes | 88 | 88 | 86 | 84 | 85 |
Illumina platforms | Illumina GA | Illumina GA | HiSeq 2000 | HiSeq 2000 | HiSeq 2000 |
Length of short paired-end reads | 75 | 75 | 90 | 90 | 90 |
Total reads (×106) | 15.18 | 13.24 | 12.77 | 12.82 | 12.81 |
Aligned reads (×106) | 14.53 | 12.58 | 12.03 | 11.45 | 11.76 |
Percentage of mapped reads (%) | 95.76 | 94.98 | 94.21 | 89.28 | 91.83 |
Mean depth | 5990× | 5127× | 6061× | 5617× | 5618× |
Number of polymorphic genes | 29 | 31 | 78 | 12 | 14 |
Number of SNPs | 36 | 39 | 724 | 38 | 26 |
Average of π (×10−3) | 0.080 (±0.232) | 0.112 (±0.260) | 1.021 (±0.815) | 0.156 (±0.404) | 0.099 (0.280) |
θ (×10−3) | 0.077 | 0.080 | 2.434 | 0.133 | 0.084 |
Population abbreviations are defined in Table 1. π, nucleotide diversity; θ, nucleotide polymorphism. Numbers in parenthesis represent the standard deviation (sd) of the π values. Polymorphic genes: the genes contain one or more SNPs in each population.