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. 2016 Jul 6;6:29272. doi: 10.1038/srep29272

Figure 2. Tissue prints were made for DTBIA from petioles of CaLas infected and healthy rough lemon and sweet orange trees.

Figure 2

Tissue prints were developed with only the secondary anti-FLAG antibody. No primary scFv labeled with FLAG were used. The secondary anti-FLAG Mab (Sigma) was conjugated with alkaline phosphatase and used at 1:10,000 dilution. Detection was with NBT/BCIP.