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. 2016 Jul 6;6:29272. doi: 10.1038/srep29272

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A secondary anti-FLAG antibody conjugated with alkaline phosphatase was used at 1:10,000 dilution without any primary scFv antibodies for specific pathogen detection. (A) CTV B2 in sweet orange (B) CTV B6 in sweet orange (C) CCDV in Citrus macrophylla (D) CaLas in sweet orange (E) X. fastidious in sweet orange (F) Healthy sweet orange. Detection was with NBT/BCIP.