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. Author manuscript; available in PMC: 2017 May 3.
Published in final edited form as: Chembiochem. 2016 Mar 18;17(9):804–810. doi: 10.1002/cbic.201500701

Figure 1.

Figure 1

Role of the NRPS system in l-ACL biosynthesis. A) Structures of the deaminocaprolactam congeners. B) HPLC chromatograms of the methanol extract from the i) wild-type strain; ii) ΔcapU mutant strain; and iii) ΔcapU mutant strain cultured with exogenously supplied l-ACL. A260, absorbance at 260 nm; asterisk (*) denotes an unidentif ied metabolite with a unique UV/Vis spectrum relative to capuramycins. C) In vitro reconstitution of l-ACL biosynthesis starting from l-Lys and apo-CapU using Svp with or without additional enzymes.