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Effect of azurin in PI3K, Akt and Src signaling. Azurin at 50 µM and 100 µM decreased phosphorylation levels of pPI3K, pAkt and pSrc in both A549 cells. Total PI3K, Akt and Src levels were also analyzed. Results are presented as the ratio of band intensity of target protein between azurin treated samples and control samples, both normalized to their respective GAPDH band intensity (* p < 0.05).
