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. 2016 May 10;15(12):1620–1630. doi: 10.1080/15384101.2016.1183846

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Figure 4. — Hxk2p is hypophosphorylated in isc1Δ cells by a Sit4p-dependent mechanism. (A) Yeast extracts were prepared from S. cerevisiae BY4741, isc1Δ and isc1Δsit4Δ cells and a phosphoproteome analysis was performed, as described in legend to Figure 1. The region of the blots showing Hxk2p and Dug1p was selected. Spot intensities were quantified by densitometry. Values are means ± SD of 3 independent experiments. *p < 0.05. (B) Chronological lifespan of S. cerevisiae hxk2Δ and isc1Δhxk2Δ cells expressing Hxk2p-S15 or Hxk2p-S15E. Cellular viability was determined as described in legend to Figure 2B. Values are means ± SD of 4 independent experiments.