Figure 5.

Snf1p activation does not mediate Hxk2p phosphorylation in sit4Δ cells. (A) Protein extracts from S. cerevisiae BY4741, sit4Δ and snf1Δ cells were analyzed by immunoblotting using anti-Snf1 or anti-AMPK (phospho T172) as described in Materials and Methods. (B) Hxk2p-GFP was immunoprecipitated from protein extracts of S. cerevisiae BY4741, sit4Δ, snf1Δ and sit4Δsnf1Δ cells and analyzed by immunoblotting using anti-GFP or anti-phosphoserine as described in Materials and Methods. Representative blots are shown (out of 3 independent experiments).