Table 1.
Saccharomyces cerevisiae strains used in this study.
| Strain | Genotype | Reference/source |
|---|---|---|
| BY4741a | Mata his3Δ1, leu2Δ0, met15Δ0, ura3Δ0 | EUROSCARF |
| sit4Δa | BY4741 sit4Δ::KanMX4 | EUROSCARF |
| cdc55Δa | BY4741 cdc55Δ::KanMX4 | EUROSCARF |
| isc1Δ | BY4741 isc1Δ::KanMX4 | EUROSCARF |
| isc1Δsit4Δ | BY4741 sit4Δ::KanMX4 isc1Δ::URA3 | 25 |
| snf1Δa | BY4741 snf1Δ::KanMX4 | EUROSCARF |
| sit4Δsnf1Δa | BY4741 sit4Δ::MXHIS3 snf1Δ::KanMX4 | This study |
| hxk2Δb, c | BY4741 hxk2Δ::KanMX4 | EUROSCARF |
| isc1Δhxk2Δb, c | BY4741 hxk2Δ::KanMX4 isc1Δ::LEU2 | This study |
| WAY.78-1 YIpHXK2-S15 | Matα ura3-52 leu 2–3,112 trp1-289 hxk1Δ::HIS3 hxk2Δ::LEU2 glk1Δ::LEU2 MAL2-8cMAL3 SUC3 YIpHXK2(S15) | 34 |
| WAY.78-1 YIpHXK2-S15A | Matα ura3-52 leu 2–3,112 trp1-289 hxk1Δ::HIS3 hxk2Δ::LEU2 glk1Δ::LEU2 MAL2-8c MAL3 SUC3 YlpHXK2(S15A) | 34 |
| WAY.78-1 YIpHXK2-S15 sit4Δ | WAY.78-1 YlpHXK2-S15 sit4Δ::KanMX4 | This study |
| WAY.78-1 YIpHXK2-S15A sit4Δ | WAY.78-1 YlpHXK2-S15A sit4Δ::KanMX4 | This study |
| BY4742 | MATα his3Δ1, leu2Δ0, lys2Δ0, ura3Δ0 | EUROSCARF |
| sit4Δa | BY4742 sit4Δ::KanMX4 | EUROSCARF |
| pph21Δpph22Δa | BY4742 pph21::KanMX4Δpph22Δ::KanMX4 | 64 |
Note. Cells harboring YEp352-HXK2-GFP (a), pRS316-HXK2-S15 (b) or pRS316-HXK2-S15E (c) are indicated with “a”, “b” and “c” superscript.