Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
A cell-cycle tailored genome editing system to enhance HDR. The NHEJ pathway dominates DSB repair in G1 and G2 whereas HDR activity peaks in mid-S phase. The Cas9-hGem(1/110) fusion protein is subjected to proteolytic destruction during G1, but its expression is restored in S/G2/M. This partially synchronizes genome editing with HDR activity and enhances rates of the latter.
