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. 2012 Nov 23;1:13–23. doi: 10.2147/ITT.S32615

Figure 4.

Figure 4

Validation of anti-IL-10 DsiRNA. NC1, HPRT, and mIL10-1 DsiRNAs were transfected into RAW264.7 cells at 10 nM, 1 nM, and 0.1 nM concentrations.

Notes: At 24 hours, cells were stimulated with 50 ng/mL LPS and RNA was harvested 8 hours later. RT-qPCR was performed and relative expression of the HPRT mRNA (in the HPRT DsiRNA transfected cultures) and IL-10 mRNA (in the IL-10-1 DsiRNA transfected cultures) normalized to the RPL23 are shown, setting the control NC1 transfection to 100%. The differences between NC1- and HPRT- or IL-10-specific siRNA are statistically significant (**P < 0.01; ***P < 0.001).

Abbreviations: IL-10, Interleukin 10; HPRT, Hypoxantine-guanine phosphorybosyl-transferase; RT-qPCR, Reverse transcriptase quantitative polymerase chain reaction.