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. Author manuscript; available in PMC: 2017 Feb 28.
Published in final edited form as: Cancer Lett. 2015 Dec 17;371(2):285–291. doi: 10.1016/j.canlet.2015.12.008

Fig. 3.

Fig. 3

Hepatitis B virus X A10/S144 double mutation prolonged G1-S transition and up-regulated the expression of p21 in a p53-independent manner. (A) HepG2 p53 null/HepG2 cells were transfected with indicated hepatitis B virus X expression vector together or control plasmid. Hepatitis B virus X, p53 and actin expression were analyzed by Western blotting. (B) Effect of hepatitis B virus X mutation on modifications of cell cycle parameters. 36 hours after transfection with indicated plasmids, cell cycle parameters were stained with propidium iodide (PI) and analyzed by flow cytometry. (C) Effect of hepatitis B virus X on cell-cycle progression. The percentage difference in the size of the G1 cell population was determined by the comparison of hepatitis B virus X expression vector transfected Hep3B/HepG2 cells with control plasmid transfected cells.