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Histochemical analysis of hand-pollinated pistils on 8th day after hand-pollination; longitudinal sections of the ovary. (A–C) Chromatin of the nuclei stained with DAPI and cell walls stained with calcofluor white. (D,E) Method combining fluorescence and differential interference contrast. (A,B) Masses of pollen tubes (arrowheads) penetrate the stigma and grow into the ovary. (C) Masses of pollen tubes (arrowheads) inside the ovary. (D) The zygote (z).
