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. 2016 Mar 11;23(1):33–43. doi: 10.1159/000443554

Fig. 3.

Fig. 3

Flow cytometric examination of apoptosis and cell cycle. a Flow cytometric examination of apoptosis, necrosis and cell viability - the Annexin V-FITC assay of C6 cells after treatment with 50 μg/ml cycloheximide (positive control), 50 μm GBP, 10 μm VPA and 10 μm TPM. Four quadrants of plots depict the following: lower left - viable cells (Annexin V-, PI-), lower right - early apoptotic cells (Annexin V+, PI-), upper right - late apoptotic cells (Annexin V+, PI+), upper left - necrotic cells (Annexin V-, PI+). b The histogram represents percentage distribution of cells in different quadrants in control and treated groups. c The histogram represents percentage distribution of cells in different phases of cell cycle in control and drug-treated groups. d Plots representing cell cycle analysis of C6 cells in control and treated groups. Black line represents control and red line represents respective treatment group.