Fig. 4.

Immunofluorescent and Western blot analysis. Immunofluorescent staining of GFAP (a-d), NFκB (e-h) and HSP70 (i-l) in control, 50 μm GBP, 10 μm VPA and 10 μm TPM-treated C6 cells. Images were captured using Nikon A1R confocal microscope. Scale bar represents the length of 50 μm. m Representative blots for GFAP, NFκB and HSP70 in control and drug-treated C6 cells. n Quantitative densitometric analysis for markers is shown in histogram. Values are expressed as mean ± SEM from 3 independent experiments. * Represents statistically significant (p < 0.05) difference in the expression of markers in treated cells with respect to control cells.