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Primary explant cultures study. Hypothalamic explants from 2-day-old pups stained for GAD (a-d), PSA-NCAM (e-h) and NCAM (i-l). Overlay images (m-p) show the co-localization of PSA-NCAM and NCAM. Images were captured using Nikon A1R confocal microscope. Nuclei were stained by DAPI. Scale bar represents the length of 50 μm.
