Table 2.

Summary of alcohol effects on EC₂₀ GABA responses of mutant ρ1 receptors. Symbols: ↑, potentiation; ↓, inhibition; —, no effect. Numbers indicate the percent change of the EC₂₀ GABA responses in the presence of alcohol (+, potentiation; −, inhibition). The colored text corresponds to the color of the amino acids in the model representations; WT, wild-type. The values for ethanol and hexanol effects correspond to the average of all WT receptors in each group. *p< 0.05, **p< 0.01, ***p< 0.001, ****p< 0.000 1 versus WT expressed in oocytes from the same batch as each mutant, as shown in the figures. Analyzed using Two-Way ANOVA with Sidak’s multiple comparisons post-hoc test.

Receptor	Ethanol	n	Hexanol	n
ρ1 WT	−42 ± 1 %	26	−27 ± 3 %	21
ρ1(T6’Y)	12 ± 2 % ****	7	4 ± 1 % ****	4
ρ1(T6’F)	14 ± 4 % ****	6	13 ± 5 % ***	6
ρ1(T6’V)	−61 ± 2 % ****	6	−96 ± 1 % ****	6
ρ1(T6’M)	−35 ± 2 %	4	−39 ± 1 % *	6
ρ1 WT	−43 ± 1 %	19	−22 ± 2 %	18
ρ1(I5’A)	−38 ± 3 %	7	−33 ± 1 % ****	7
ρ1(I5’V)	−50 ± 4 %	4	−36 ± 1 %	4
ρ1(I5’F)	−40 ± 3 %	4	−37 ± 2 % **	4
ρ1(I5’W)	−57 ± 2 % **	5	−52 ± 2 % ****	5