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Residual antioxidant capacity in amidomethylated Mfp-6. (A) Free sulfhydryl groups in Mfp-6 were labeled at pH 3 using 2-iodoacetamide (IAM). (B) Time course of 100 µM DPPH radical quenching using 2.5 µM pure (■) or thiol group-blocked (●) Mfp-6.