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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Antiviral Res. 2016 Apr 21;131:100–108. doi: 10.1016/j.antiviral.2016.04.001

Figure 1. Induction of cross-reactive antibodies to group 1 and group 2 HA proteins.

Figure 1

Mice (5/grp) were immunized with either PBS, or 2 different strains of rHA derived from H1CA A/California/07/09 (H1N1) [CA07] or A/VietNam/1203/2004 (H5N1) [VN1203] and adjuvanted with GLA-SE. Sera were assayed by ELISA for binding to rHA proteins derived from (a) CA07, (b) VN1203, (c) A/Indonesia/05/2005 (H5N1) [Indo05]), (d) A/Aichi/2/1968 (H3N2) [Aichi68], and (e) A/Shanghai/1/2013 (H7N9) [Shanghai13]. As indicated, sera from the CA07-immunized mice bound both rH5 proteins and to a lesser extent rHA from Aichi68 or Shanghai13. Similarly, sera from VN1203-immunized mice effectively bound rHA from Indo05 and CA07, and reduced binding to Aichi68 or Shanghai13.