Table 1.
cDNA and Murine IgVH Primers.
| a. cDNA primers | ||
| 1 | IgG1 | ACAAGTACATTTCCTCGTCCT ANCNTNCNANCN CMCAGGGGCCAGTGGATA |
| 2 | IgG2 | ACAAGTACATTTCCTCGTCCT ANCNTNCNANCN BCCAGGGACCAAGGGATA |
| b. IgVH Forward PCR primers | ||
| Pool | Primer # | Sequence* |
| 1 | 1.1 | GATAGAGCTGCAGCTTGTTGAGTCTG |
| 1.2 | GATAGAGCTTCAGCTCCAGCAGTCTG | |
| 1.3 | GATAGAGCTGAAGCTTCTCCAGTCTG | |
| 1.4 | GATAGAGCTYCAGCTGCAGCAGTCTG | |
| 2 | 2.1 | GATAGAGCGCTGAGCTKGTGAAGCCT |
| 2.2 | GATAGAGCSCTGAGCTGGTGAAGCCT | |
| 3 | 3.1 | GATAGAGCCTGGCCCTGGKATATTGC |
| 4 | 4.1 | GATAGAGCGTGCACATTGACACAGAG |
| 4.2 | GATAGAGCAGTGCAGCCCTCACAGAG | |
| 5 | 5.1 | GATAGAGCGAACTGTGGTGGTGAAAC |
| 5.2 | GATAGAGCCTGGAGGGTCCCTGAAAC | |
| 5.3 | GATAGAGCGAGCTGAACTGGTGAAAC | |
| 5.4 | GATAGAGCACGCTGAGTTGGTGAAAC | |
| 5.5 | GATAGAGCCTGGACAGTCCCTGAAAC | |
| 6 | 6.1 | GATAGAGCGTGAGGCCTGGAAATTCT |
| 7 | 7.1 | GATAGAGCCTTCAGGAGTCAGGACCT |
| 7.2 | GATAGAGCCTGCARCAGTCTGGACCT | |
| 7.3 | GATAGAGCMTGAAGGAGTCAGGACCT | |
| c. IgVH Reverse PCR Primer | ||
| LP4 | ACAAGTACATTTCCTCGTCCT | |
*
C, B, K, Y, R, S, or M encode ambiguous nts based on the universal IUPAC Nucleotide Coding System