Table 6.
Functional analysis of head-binding IgG MAbs.
| a. Screening ELISA profile (1450) | b. HI Titer (H5 clades) (ng/uL) | c. Nature of Epitope | ||||||
|---|---|---|---|---|---|---|---|---|
| MAbs | H5VN | H1CA | HA1 | A/VietNam (clade 1) | A/Cambodia (clade 1) | A/Turkey (clade 2.2) | A/Anhui (clade 2.2.4) | Linear or Conformational |
| 808K | 2.918 | nb | 2.999 | 2.07 | 2.07 | 2.07 | NA | Linear |
| 24415K | 3.129 | nb | 3.269 | 1.64 | 1.64 | 1.64 | NA | Linear |
| 2407K | 3.099 | nb | 3.057 | 0.43 | 0.87 | 0.43 | NA | Linear |
| 1620K | 2.599 | nb | 2.931 | 0.94 | 0.94 | 0.00 | NA | Conformational |
| 1968K | 3.003 | nb | 2.951 | 3.13 | 3.13 | 3.13 | NA | Linear |
| 491K | 2.704 | nb | 3.102 | 1.72 | 0.86 | 13.75 | NA | Conformational |
| 1166K | 2.844 | nb | 3.067 | 0.82 | 1.64 | 1.64 | NA | Linear |
| 447K | 2.245 | nb | 2.921 | 6.88 | 0.86 | 0.00 | NA | Conformational |
| 632G | 2.779 | nb | 2.612 | NA | NA | NA | NA | Conformational |
nb = no binding; NA = no HI activity