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. Author manuscript; available in PMC: 2016 Jul 6.
Published in final edited form as: Invest New Drugs. 2014 Nov 23;33(1):86–94. doi: 10.1007/s10637-014-0185-3

Figure 4. Flow cytometric analysis of pathways involved in the regulation of NFκB in AsPC-1 cells treated with microsclerodermin A.

Figure 4

AsPC-1 cells were treated with either 2.4 μM microsclerodermin A or methanol (vehicle control) for 6 hours and analyzed via flow cytometry to determine changes in expression of a) Iκκα and TNFAIP3, which keep NFκB in its inactive form in the cytoplasm; b) the members of the Toll-like Receptor signaling pathway TLR-1, TLR-3, IRAK and phosphorylated Elk; c) the TNF-related apoptosis-inducing ligand (TRAIL). No significant changes were observed in these pathways in cells treated with microsclerodermin A compared to cells treated with vehicle control. Graphical representation of the flow cytometry data shows the average of 3 experiments. Error bars represent standard deviation.