Figure 3.

Schematic of dissociation protocol. A) Wash larvae and dissect in SSM. B) Collect imaginal discs in Dulbecco's PBS in a glass dish. C) Replace PBS with TrypLE, cover with Petri dish lid and secure with tape. D) Incubate in metal bead bath for 15 min at 37°C. E) Stop enzyme action by flooding with SSM. F) Further dissociate cells by pipetting 2–3 times with a 1000 μL pipet tip. F) Pass cells through a cell strainer to remove any remaining aggregates.