FIG 5.
Host adaptive immune response in the local draining lymph nodes. (A) Generation of an adaptive cellular immune response in the local draining (mandibular) lymph nodes (MLN) was assessed by flow cytometry to evaluate expansion of CD4+ and CD8+ T cells following ocular HSV-1 infection (n = 9 to 10 mice/group; 3 independent experiments). (B) The total CD8+ T cell populations shown in panel A were subgated to phenotypically characterize the effector memory (TEM; CD44+ CD62L−) or central memory (TCM; CD44+ CD62L+) subsets and quantify activated (CD69+) cells. (C) The total CD4+ T cell populations shown in panel A were also subgated to phenotypically characterize the TEM and TCM subsets. Representative flow plots are shown for each group. Significance thresholds for all panels are indicated as follows: */^, P < 0.05; **/^^, P < 0.01; ***/^^^, P < 0.001. Significant differences in results relative to the 0ΔNLS- and the gD-2-immunized groups are shown as asterisks and carets, respectively. Green dashed lines signify the average UI value for each population.