FIG 5.
Atg7 can have pro- or antiviral activity in Drosophila. (A) Schematic representation of the Atg7 locus and the deletion mutants used. Exons are indicated by rectangles with the coding region highlighted in orange. Deletions are indicated by the gaps in the blue lines. (B and D) Survival of Atg7 mutant (Atg7d14/Atg7d77) and control (Atg7d14/CG5335d30) flies after VSV (B) or FHV (D) infection. Tris or conditioned medium (CM) was used as an injection control. (C and E) Quantitative RT-PCR analysis of the accumulation of viral RNA at the indicated day postinfection in control (Atg7d14/CG5335d30) and Atg7 mutant (Atg7d14/Atg7d77) flies injected with VSV (C) or FHV (E). Data represent the means ± standard errors (B and D) or SD (C and E) of three independent experiments, each containing three groups of 10 (B and D) or 6 (C and E) flies. ns, not significant; *, P < 0.05; ***, P < 0.001 (log rank [B and D] or unpaired t test [C and E]).