FIG 3.

SBV-S32 attenuation maps to nucleotide 167/142 of the nucleocapsid/NSs gene. (A) Schematic representation displaying the positions of nucleotide changes of SBVp32 compared to wild-type SBV in the nucleocapsid and NSs open reading frames. Asterisks indicate nonsynonymous changes. (B) Survival plots of 7-day-old mice inoculated intracerebrally with 400 PFU of the indicated reassortants. Asterisks indicate significance levels (*, P ≤ 0.05 [as determined by a log rank test and a Mantel-Cox test]). (C) Replication kinetics of SBV S point mutants in CPT-Tert cells and primary sheep fibroblasts. The graph displays the averages of data from two and four independent experiments, respectively.