TABLE 2.
Primer for generating SIA binding defective mutants and titer quantification
| Primer (VP aa)a | Sequence (5′–3′) |
|---|---|
| N447S-F | CCAATACCTGTATTACCTGAGCAGAACTCAAAATCAG |
| N447S-R | CTGATTTTGAGTTCTGCTCAGGTAATACAGGTATTGG |
| S472R-F | CTCCAGCTGGCATGCGGGTTCAGCCCAAAAACTG |
| S472R-R | GGGCTGAACACGCATGCCAGCTG |
| V473D-F | GCTGGCATGTCTGACCAGCCCAAAAACTGGC |
| V473D-R | CAGTTTTTGGGCTGATCAGACATGCCAG |
| N500E-F | CAGACAACAACAACAGCGAATTTACCTGGACTGGTGCTTC |
| N500E-R | GCACCAGTCCAGGTAAATTCGCTGTTGTTGTTG |
| T502S-F | CAACAACAGCAATTTTTCGTGGACTGGTGCTTC |
| T502S-R | TTTTGAAGCACCAGTCCACGAAAAATTGCTGTTGTTGTTG |
| W503A-F | CAACAGCAATTTTACCGCTACTGGTGCTTCAAAATATAACC |
| W503A-R | GGTTATATTTTGAAGCACCAGTAGCGGTAAAATTGCTGTTG |
| Luciferase-F | ATTACACCCGAGGGGGATGA |
| Luciferase-R | CTCTCACACACAGTTCGCCT |
a
aa, amino acid.