FIG 3.

HSV-1-pUL36-WD/AA-WE/AA does not affect the expression pattern of pUL36 proteins. Vero cells (A) and Vero-HS30 cells (B) were mock infected or infected synchronously with the indicated HSV-1 strains and harvested at 12 hpi. The proteins were separated in gradient polyacrylamide gels and probed with antibodies directed against pUL36 (pAb 147; residues 1408 to 2112), pUL36 (pAb 3; residues 936 to 951), pUL37 (pAb anti-pUL37), VP26 (pAb anti-VP26), CheVP26 (MAb anti-red fluorescent protein), or as loading control actin (MAb 1501).