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. 2016 May 12;90(11):5368–5383. doi: 10.1128/JVI.03167-15

FIG 4.

FIG 4

Accumulation of cytosolic capsids and reduced secondary envelopment in cells infected with HSV-1(17+)Lox-pUL36-WD/AA-WE/AA. Vero cells were infected synchronously with HSV-1(17+)Lox (A) or HSV-1(17+)Lox-pUL36-WD/AA-WE/AA (B to G) PFU, fixed at 14 hpi, and analyzed by electron microscopy. The inset in panel A contains three consecutive sections showing viral genomes within cytoplasmic capsids. A white asterisk indicates the microtubule-organizing center, and N indicates the nucleus. The primary enveloped virion (C), cytosolic capsids (D), wrapping intermediate (E), enveloped virus particle (F), and extracellular virions (G) were assembled upon infection with HSV-1-pUL36-WD/AA-WE/AA. Scale bars, 200 nm.