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. 2016 May 12;90(11):5368–5383. doi: 10.1128/JVI.03167-15

FIG 5.

FIG 5

HSV-1-pUL36-WD/AA-WE/AA accumulates capsids in the perinuclear region and recruits pUL36. Vero cells were infected synchronously with HSV-1(17+)Lox-CheVP26 (A), HSV-1(17+)Lox-CheVP26-WD/AA-WE/AA (B), or HSV-1(17+)Lox-CheVP26-ΔUL36 (C) and fixed at 10 hpi. The specimens were labeled for pUL36 (pAb 147; white in panels ii and v and green in panels iii and vi) and analyzed by confocal microscopy. The capsids were visualized by fusion protein CheVP26 (white in panels i and iv and red in panels iii and vi). The nuclei were identified by DAPI labeling and are depicted by dashed white lines. Scale bars, 3 μm.