TABLE 1.
HSV-1(17+)-pUL36-WD/AA-WE/AA is impaired in secondary capsid envelopmenta
| Exp no. and HSV-1(17+) type | No. of cells or snapshotsb |
No. of capsids |
Area (1,000 μm2) |
No. of capsids/area (1/1,000 μm2) |
% of cytoplasmic capsids |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Nuclear | Cytoplasmic | Nuclear | Cytoplasmic | Nuclear | Cytoplasmic | Nuclear | Cytoplasmic | Cytosolic | Wrapping intermediates | Enveloped virions | |
| Exp 1, Lox | 11* | 11* | 940 | 715 | 1.9 | 3.5 | 493 | 204 | 42 | 32 | 26 |
| Exp 2, Lox | 22* | 84# | 1,362 | 910 | 3.5 | 1.9 | 387 | 479 | 51 | 26 | 22 |
| Exp 2, Lox-CheVP26 | 20* | 101# | 1,541 | 908 | 4.3 | 2.0 | 356 | 454 | 54 | 26 | 20 |
| Total or avg | 53* | 11*; 185# | 3,843 | 2,533 | 9.7 | 7.4 | 412 | 379 | 49 | 28 | 23 |
| Exp 1, Lox-WD/AA-WE/AA | 10* | 10* | 920 | 552 | 2.1 | 3.2 | 446 | 173 | 70 | 17 | 13 |
| Exp 2, Lox-WD/AA-WE/AA | 22* | 92# | 905 | 1,867 | 3.2 | 1.9 | 279 | 983 | 84 | 12 | 4 |
| Exp 2, Lox-CheVP26-WD/AA-WE/AA | 20* | 100# | 1,262 | 1,720 | 3.5 | 2.2 | 362 | 782 | 86 | 9 | 4 |
| Total or avg | 52* | 10*; 192# | 3,087 | 4,139 | 8.8 | 7.3 | 362 | 646 | 80 | 13 | 7 |
a
Vero cells infected with HSV-1(17+)Lox, HSV-1(17+)Lox-pUL36-WD/AA-WE/AA, HSV-1(17+)Lox-mCheVP26, or HSV-1(17+)Lox-mCheVP26-pUL36-WD/AA-WE/AA were analyzed by electron microscopy, and the numbers of nuclear capsids, cytosolic capsids, wrapping intermediates, and enveloped virions were determined.
b
Shown are the number of cells of which the entire nucleus or the entire cytoplasm had been imaged (*) or the number of snapshots from a cytoplasmic region (#).