FIG 7.

During entry, acid phosphatases dephosphorylate the surface residues associated with nuclear export potential. (A) A9 mouse fibroblasts (3 × 10⁶) were infected with purified FC-P₂ (5,000 particles per cell) at 4°C. After the removal of unbound viruses, cells were incubated at 37°C for the indicated times and were subjected to AEX-qPCR. In order to inhibit acid phosphatases, 150 nM BafA₁ was added 15 min prior to virus internalization at 37°C. (B) Virus progenies with equivalent amounts of FC-P₁ and FC-P₂ were treated with CHT alone or with CHT plus phosphatase and were analyzed by AEX-qPCR.