FIG 1.

KSHV ORF52 is expressed with late gene kinetics. (A, B) Anti-ORF52 mouse monoclonal antibodies (4H4 and 8C8) specifically recognize ORF52 protein in both transiently transfected cells (A) and induced BCBL-1/iSLK.219 cells (B). (C) The two anti-ORF52 monoclonal antibodies were used for epitope mapping by Western blot analysis, using purified glutathione S-transferase (GST)-ORF52 proteins encompassing a series of 10-aa internal deletion mutants. (D) iSLK.BAC16 cells were induced in the presence or absence of PAA for the indicated times (days postinduction [dpi]). Viral protein levels were assessed by Western blotting with antibodies to the indicated proteins. IE, immediate early gene; E, early gene; L, late gene.