FIG 3.

ORF52 is a virion protein. (A) Cell lysates of uninduced and induced iSLK.BAC16 cells (lanes 1 and 2) and gradient-purified KSHV virions (lane 3) were resolved by SDS-PAGE and analyzed by Western blotting with antibodies to the indicated proteins. (B) KSHV virions were fractionated on a 20-to-60% sucrose gradient. Fractions and input were analyzed by Western blotting with antibodies to the indicated proteins. (C) Viral DNA of each fraction in shown in panel B was extracted and analyzed by quantitative PCR (qPCR) (red), and the percentage of sucrose of each fraction was calculated from the refractive index (green). The two values were plotted for each fraction collected.