(A) Peaks and their overlaps before and after SAHA treatment in coding regions for acetylated histones and BRD4 in BT474.
(B) Peaks and their overlaps before and after SAHA treatment in intergenic regions for acetylated histones and BRD4 in BT474. Number of peaks in each category is listed. Only peaks present in both replicates were considered.
(C) Within intergenic BRD4 binding sites, those that are lost upon SAHA, stay bound, and are gained after SAHA are analyzed for intensity of BRD4 binding changes.
(D) Acetylation changes at intergenic BRD4 binding sites.
See also Figure S4 for the overlap of genes affected by TSA, SAHA, JQ1 (BRD4 inhibitor), and FLAVO treatments, and JQ1 effects on ERBB2 and MYC expression and elongation.