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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Free Radic Biol Med. 2016 Mar 4;95:43–54. doi: 10.1016/j.freeradbiomed.2016.02.032

Figure 4. Mutant parasites show no changes in the levels of mtDNA damage or mtDNA abundance.

Figure 4

(A) Upper panel, representative gel showing the amplification of a 5.7 kb mtDNA fragment from WT, pbggcs-ko and pbgr-ko parasites. Lower panel, relative amplification of a 5.7 kb mtDNA fragment that was normalized for changes in mtDNA abundance. (B) Upper panel, representative gel showing the amplification of the 186 bp mtDNA fragment. Lower panel, relative amplification of a 186 bp mtDNA fragment representative of mitochondrial abundance. No significant differences in mtDNA damage or mtDNA abundance were observed between WT and mutant parasites. Bars represent the SEM from 3 independent experiments, and 14 and 12 QPCR analyses for mtDNA damage or mtDNA abundance, respectively.