Fig 3. High titres of R5-tropic HIV.EGFP reporter virus are needed to infect resting CD4+ T-cells.

Resting CD4+ T-cells from 4 donors were labelled with eFluor670 proliferation dye and cultured without (red) or with autologous mDCs at 1 mDC:10 T-cell ratio (blue) for 24 hours. Cells were incubated with increasing TCID₅₀ per cell of HIV^{NL4.3(AD8)-EGFP} for 2 hours and cultured for 5 days in a low concentration of IL-2 (2 U/ml). 5 days post-infection, cultures were analysed for EGFP expression by flow cytometry to quantify productive infection (A). Non-proliferating eFluor670^hiEGFP- cells were also sorted and cultured for 3 days with anti-CD3/anti-CD28 plus IL-7 to induce EGFP expression from latent infection (B). The percentage of EGFP+ cells per 10⁴ cultured cells is shown. Columns represent the median of donors tested with each donor shown as a different symbol.