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. 2016 Jul 6;11(7):e0158839. doi: 10.1371/journal.pone.0158839

Fig 1. FAK contributed to IL-6/sIL-6R-induced VEGF-C expression in SV-LECs.

Fig 1

(A) Cells were pretreated with the vehicle or NSC 667249 (0.3 μM) for 30 min before treatment with IL-6 plus sIL6R (20 ng/ml) for another 24 h. The VEGF-C level and was then determined by immunoblotting. Each column represents the mean ± S.E.M. of eight independent experiments. (B) After treatment as described in (A), cell culture media were collected and VEGF-C in the media was quantified using a ELISA kit. Each column represents the mean ± S.E.M. of four independent experiments. (C) Cells were transiently transfected with VEGF-C promoter-luc-370 and renilla-luc for 48 h. After transfection, cells were treated with vehicle or NSC 667249 (0.3 μM) for 30 min, followed by treatment with IL-6 plus sIL6R (20 ng/ml) for another 24 h. Luciferase activity was then determined as described in the “Materials and Methods” section. Data represent the mean ± S.E.M. of three independent experiments performed in duplicate. (D). Cells were pretreated with the vehicle or NSC 667249 (0.3 μM) for 30min followed by treatment with IL-6 plus sIL6R (20 ng/ml) for another 30 min. The extent of FAK phosphorylation was then determined by immunoblotting. Each column represents the mean ± S.E.M. of four independent experiments. *p<0.05, compared to the control group; # p<0.05, compared to the vehicle-treated group in the presence of IL-6 plus sIL6R.