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. 2016 Jul 6;11(7):e0156971. doi: 10.1371/journal.pone.0156971

Fig 1. A representative analysis of a selection of TAAs by Western blot (WB) and Silver stain.

Fig 1

Samples were run on 4–20% precast polyacrylamide gels with a broad range protein markers. The 4 antigens (Gankyrin, H-Ras, RalA, and HCC1) were then either probed with anti-His antibody (A) or silver stained (B). Silver stain was used to check the purity of the antigens. The expected molecular weight of the examined antigens, Gankyrin, H-Ras, RalA, and HCC1 are 25, 40, 22 and 64 kDa respectively. The similar bands obtained before and after buffer exchange by both WB and silver stain indicate that the buffer exchange technique had no effect on the integrity of the antigens. C: Silver stain of 4 TAAs: Annexin, P53, KRAS and RAF1 (the expected molecular weight are 39, 53, 57 and 73 kDa respectively).