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. 2016 Jun 29;9:3939–3949. doi: 10.2147/OTT.S105245

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© 2016 Xing et al. This work is published and licensed by Dove Medical Press Limited

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Western blot analysis of the ERK/MEK signaling pathway.

Notes: Recombinant CXCL16 could induce increased E-cadherin, and decreased p-ERK, p-MEK, MMP2/9, and N-cadherin. However, ERK and MEK showed no changes. β-actin was used as an internal loading control. Treg: untreated Tregs; Treg-C: Tregs treated with recombinant CXCL16; Treg-C+S: Tregs treated with recombinant CXCL16 and CXCR6 siRNA; GIST-T1: untreated GIST-T1 cells; GIST-T1-C: GIST-T1 cells treated with recombinant CXCL16; GIST-T1-C+S: GIST-T1 cells treated with recombinant CXCL16 and CXCR6 siRNA; GIST882: untreated GIST882 cells; GIST882-C: GIST882 cells treated with recombinant CXCL16; GIST882-C+S: GIST882 cells treated with recombinant CXCL16 and CXCR6 siRNA.

Abbreviation: GIST, gastrointestinal stromal tumors.