Fig. 3.

Effect of altering CADM1 expression on insulin secretion by primary rat islet cells. A: dispersed rat islets were transfected with a CADM1-expressing plasmid or mock-transfected (Mock) with empty vector and then incubated for 48 h. Insulin secretion was then measured during a 1-h incubation in 16.7 mM glucose with 0.1 mM IBMX. B: dispersed rat islets were transfected for 72 h with pool of either scrambled (Scr) or CADM1 siRNA. Insulin secretion was measured over a 1-h incubation in 16.7 mM glucose with 0.1 mM IBMX. Total insulin secreted is shown normalized to total cellular insulin content. C: to verify effectiveness of CADM1 siRNA, CADM1 mRNA was measured by RT-qPCR. CADM1 mRNA level after treatment with CADM1 siRNA is shown relative to level after treatment with control (scrambled) siRNA. Data are represented as the mean ± SE of 3 experiments. *P < 0.05; **P < 0.01; ***P < 0.005.