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. 2016 Apr 12;310(11):E874–E885. doi: 10.1152/ajpendo.00318.2015

Fig. 5.

Fig. 5.

Insulin secretion by β-cells cocultured with COS-7 cells expressing CADM1. A and B: INS-1 cells were cocultured (CCx) with COS-7 cells pretransfected with CADM1 expression vector (black bars) or mock-transfected (mock) with empty (control) vector (open bars). After 24 h, the cocultures were incubated for 1 h in 2.75 mM (low) glucose or in 16.7 mM (high) glucose with 0.1 mM IBMX (A) or in 2.74 mM glucose supplemented with either 0 (−) or 30 mM (+) KCl (B). Insulin secreted during this last hour is shown as %cellular insulin content. C: islets were isolated from male Sprague-Dawley rats and dispersed. Islets were then cocultured with COS-7 cells as in A for 24 h, followed by 1-h incubation in low or high glucose with 0.1 mM IBMX. D: human islets were dispersed and then cocultured as in A for 24 h, followed by 1 h incubation in low or high glucose with 0.1 mM IBMX. Insulin secreted was normalized to total cellular insulin content. All data are represented as means ± SE from 6 samples assayed in duplicate and representative of 3 experiments. *P < 0.05; **P < 0.01; ***P < 0.005.