Fig. 1.

Evolutionary conservation of C1q/TNF-related protein 5 (CTRP5) in vertebrates and its tissue expression profile in humans. A: sequence alignment of human (NP_001265360), mouse (NP_001177248), chicken (XP_001232467), frog (Xenopus; XP_002935065), and zebrafish (NP_001025124) CTRP5 using a web-based Clustal W (version 2) tool (26). Identical amino acids are shaded black, and similar amino acids are shaded gray. Shading was done using the web-based BoxShade tool. The NH₂-terminal signal peptide collagen domain (with G-X-Y repeats) and the COOH-terminal globular C1q domain are indicated. B: quantitative real-time PCR analysis of human CTRP5 mRNA expression across 47 tissue types. Expression levels of CTRP5 in each tissue were normalized to GAPDH.