Fig. 5.

Taxonomic changes in the fecal microbiome associated with repeated exercise. Average relative abundance of the major phyla (A) and genera (B) in feces of HFD-fed untrained and exercise-trained mice. Depiction of the only significantly different genus-level change [Bacteriodales (o)] in the feces (C). Alpha diversity (Shannon index) in exercise-trained vs. untrained vs. PreTreadmill conditions in feces from HFD-fed mice (D). Relative Bacteroidetes/Firmicutes ratio in exercise-trained vs. untrained vs. PreTreadmill conditions in feces from HFD-fed mice (E). A and B: data are mean values; C: data are box-and-whisker plots; D and E: data are means ± SE. #P < 0.05 Trained vs. Untrained. *P < 0.05 Trained vs. PreTreadmill. Ttaxonomic data for the PreTreadmill condition are presented in Fig. 6. Nos. of mice analyzed for each condition are shown in brackets.