Food intake, physical activity, and energy expenditure changes in 12-wk-old female mice on standard rodent chow. Hour 0 represents the beginning of lights-on; n = 7–9/group. A: food intake over 24 h. Genotype: F(2, 3,842) = 10.28, P < 0.0001; time: F(47, 3,842) = 3.594, P < 0.0001; interaction: F(94, 3,842) = 1.361, P = 0.0124. B: food intake during the light phase, F(2, 20) = 9.791, P = 0.001; dark phase, F(2, 20) = 5.956, P = 0.0093; both phases, F(2, 20) = 16.65, P < 0.0001. C: locomotor activity defined as the sum of ambulatory (break of 2 adjacent beams in succession) and fine (break of the same beam twice) activity over 24 h. Genotype: F(2, 3,888) = 54.14, P < 0.0001; time: F(47, 3,888) = 16.03, P < 0.0001; interaction: F(94, 3,888) = 1.741, P < 0.0001. D: locomotor activity during the light phase, F(2, 20) = 7.541, P = 0.0036; dark phase, F(2, 20) = 3.555, P = 0.0477; both phases, F(2, 20) = 5.853, P = 0.01. E: resting energy expenditure relative to lean mass; slope P = 0.458, intercept P = 0.171. F: respiratory exchange ratio, F(2, 20) = 25.67, P < 0.0001. Effects of genotype are indicated by horizontal lines terminating in vertical lines. *P < 0.05, comparison between control and ABKO; #P < 0.05, comparison between control and LABKO; +P < 0.05, comparison between ABKO and LABKO in A and C; **, ##, and ++P < 0.01; ***, ###, and +++P < 0.001; **** and ++++P < 0.0001.