Table 1.
A549 Conditioned medium | t = 6 h |
t = 24 h |
|||||
---|---|---|---|---|---|---|---|
IL-6 | MIP-2 | MMP-9 | IL-6 | MIP-2 | MMP-9 | ||
2% | C | BDL | BDL | BDL | BDL | BDL | BDL |
T | 327.3 ± 77.5* | 25.4 ± 7.6* | 29.3 ± 3.2* | 780.8 ± 104.5*† | 84.2 ± 11.7*† | 60.2 ± 18.0*† | |
5% | C | BDL | BDL | BDL | BDL | BDL | BDL |
T | 734.3 ± 65.7* | 60.1 ± 10.4* | 49.2 ± 9.7* | 1134.6 ± 244.8*† | 105.1 ± 23.6*† | 90.5 ± 14.3*† | |
15% | C | BDL | BDL | BDL | BDL | BDL | BDL |
T | 1051.7 ± 301.4* | 201.3 ± 30.5* | 66.5 ± 15.1* | 2244.3 ± 280.1*† | 353.2 ± 26.9*† | 138.3 ± 66.1*† | |
25% | C | BDL | BDL | BDL | BDL | BDL | BDL |
T | 1945.8 ± 206.9* | 362.2 ± 35.9* | 203.1 ± 14.8* | 3175.8 ± 301.4*† | 585.3 ± 22.1*† | 511.8 ± 36.2*† | |
50% | C | BDL | BDL | BDL | BDL | BDL | BDL |
T | 3608.6 ± 205.2* | 521.8 ± 20.6* | 353.9 ± 39.2* | 4703.6 ± 217.7*† | 799.5 ± 17.2*† | 603.4 ± 30.9*† |
C: control (neural cells treated with non-stimulated A549 medium, as explained in Methods); T: treated (with LPS-stimulated A549 2%, 5%, 15%, 25%, or 50% A549 conditioned medium). Results are expressed as mean ± SD; n = 9. Each T group is compared with its corresponding C group. Levels are expressed as picograms per milliliter. BDL: below the detection limit of the assay; *P < 0.0001 respect to each control group, †P < 0.05 t = 24 h vs. t = 6 h.