Fig. 2.

Eighteen-hour exposure to anion- and cation-channel blockers leads to increased LRRC8A protein expression in wild-type A2780 cells. Wild-type A2780 cells were exposed to increasing concentrations of the anion- and cation-channel blockers (NS3782, DIDS, and Clofilium) for 18 h. A: lysates were taken and used for Western blot analysis using a monoclonal antibody raised against human LRRC8A and β-actin (loading control). B: LRRC8A/β-actin protein band intensity ratios were calculated from Western blots shown in A and values given relative to control cells. Data represent mean of 7 sets of experiments ± SE. # and *: Significantly increased compared with Control without inhibitor when tested by ANOVA, Fisher LSD method and Student's t-test, respectively.