Fig. 6.

Cell viability of A2780 after Cisplatin treatment is determined by LRRC8A. The cell viability in A2780WT and A2780CisR was measured by MTT (see experimental procedures). A: A2780CisR cells were exposed to 10 μM Cisplatin for 48 h and A2780WT cells were likewise treated with Cisplatin in the absence or presence of 400 μM DIDS. Data represent 5 (A2780WT) and 3 (A2780CisR) individual sets of experiments ± SE. * and #: Significantly decreased by Cisplatin compared with the respective control and significantly increased compared with Cisplatin treatment in the absence of the inhibitor, respectively (Student's t-test). B: A2780WT and A2780CisR were transfected with either an empty vector or a vector carrying the LRRC8A gene-information. Following 1 day transfection, half of the cells were treated with 10 μM Cisplatin and left for another 48 h before measuring the viability. Data represent 6 (A2780WT) and 4 (A2780CisR) individual sets of experiments ± SE. * and #: Significantly decreased by Cisplatin and vector expression compared with the respective control, respectively (Student's t-test). §Significantly reduced by LRRC8A expression compared with empty vector expression (Student's t-test).