Fig. 9.

LRRC8A expression in the plasma membrane of wild-type and cisplatin-resistant A549 cells. Proteins exposed to the extracellular compartment in A549WT and A549CisR10 cells were biotinylated, cells lysed, and biotinylated proteins extracted and identified by Western blot as indicated in experimental procedures. A: representative blots where histone (H3) was used to verify the absence of intact cells in the preparation and Na⁺/K⁺-ATPase was used as loading control. B: protein ratio of LRRC8A/Na⁺/K⁺-ATPase was calculated and given relative to the ratio in A549WT cells. Values are mean of 3 sets of separate experiments. *Significantly reduced compared with A549WT (Student's t-test).