Fig. 1.
Rab25 regulates surface abundance of CaV1.2 channels. A: representative Western blots showing effect of scrambled, Rab4A, Rab11A, or Rab25siRNA on levels of indicated Rab proteins in cerebral arteries. B: mean data demonstrating the reduction in total Rab4, Rab11A and Rab25 proteins in arteries treated with Rab4A, Rab11A, or Rab25siRNAs, respectively compared with scrambled controls (n = 5 for each). C: representative Western blots illustrating effects of Rab4A and Rab11A siRNAs on surface and intracellular CaV1.2 protein. Blots illustrate both the full-length 240-kDa and truncated 190-kDa CaV1.2 subunits that are present in arterial myocytes (2). D: representative Western Blots showing effects of Rab25 siRNA on surface and intracellular CaV1.2 protein expression. E: mean data illustrating regulation of surface and intracellular CaV1.2 protein levels by Rab4A, Rab11A, and Rab25 knockdown normalized to scrambled controls (n = 6 for Rab4A knockdown, n = 3 for Rab11A knockdown, n = 4 for Rab25 knockdown). F: Rab4A, Rab11A, and Rab25 siRNAs do not alter CaV1.2 channel cellular distribution calculated as the percentage of total Ca2+ located at the cell surface (n = 6 for Rab4A knockdown, n = 3 for Rab11A knockdown, n = 4 for Rab25 knockdown). *P < 0.05, compared with scrm control.