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. 2016 Apr 20;310(11):C983–C992. doi: 10.1152/ajpcell.00059.2016

Fig. 4.

Fig. 4.

PDGF induces EGR1 binding to the SphK1 promoter in hPASMCs. A: ChIP real-time PCR analysis demonstrates EGR1 binding to the EGR1-“B” site within the SphK1 promoter following stimulation of hPASMC with PDGF (20–100 ng/ml, 1 h), with optimized chromatin digestion of ChIP samples (B). C and D: representative DNA gel electrophoresis of ChIP PCR products demonstrates enhanced EGR1 binding to the EGR1-“B” site within the SphK1 promoter following stimulation of hPASMC with PDGF (20–100 ng/ml, 1 h), with quantification of data signal relative to input. Results are shown as means ± SE from at least 3 experiments. **P < 0.01, ***P < 0.001 vs. IgG control unless otherwise indicated.